PRIMER Designing for Gene Cloning and Expression Analysis
Enter the Gene ID or NCBI ID to fetch the target sequence and then paste the nucleotide sequence in the Primer design Template section and get the set of primers just clicking Pick primers.
Press Search to fetch the genomic sequence, then paste it into Primer3Plus below to design cloning primers.
Primer3Plus — Paste the sequence above to design primers
Open in new tab
Untergasser A. et al. Primer3—new capabilities and interfaces.
Nucleic Acids Research 40(15), e115 (2012).
doi:10.1093/nar/gks596
Press Search to submit to NCBI Primer-BLAST (may take ~80 seconds).
Ye J. et al. Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction.
BMC Bioinformatics 13, 134 (2012).
doi:10.1186/1471-2105-13-134
